Fig. 6

Expression of DlRan genes under 2, 4-D treatment. Embryogenic calli were treated with M0 supplemented with 0.5, 1.5 and 2.0 mg/l of 2,4-D and 2,4-D free medium, respectively. RNA was extracted from embryogenic calli and analyzed by realtime PCR to determine the relative abundance of DlRan genes. a Abundance of N type DlRan transcripts, b abundance of D type DlRan transcripts. Abundance was normalized to Fe-SOD and EF-1a. Significance was tested by one-way ANOVA using SPSS 13.0. Different letters above the bars indicate significant differences according to the least significant difference test at 5 % level. Data are mean ± SE (n = 3)