Fig. 5

Relative expression levels of DlRan genes during longan somatic embryogenesis determined by qRT-PCR. Expression level was normalized to Fe-SOD and EF-1a. Data are mean ± SE (n = 3). a Expression level of N type DlRan transcripts (DlRan3B-1–DlRan3B-9, DlRanC-1–DlRan3C-3, DlRanD-1and DlRanD-2). b Expression level of D type DlRan transcripts (DlRan3A-1–DlRan3A-14, DlRanE-1, DlRanF-1 and DlRanG-1). EC friable-embryogenic callus, EC II embryogenic callus II, ICpEC incomplete compact pro-embryogenic cultures, CpECGE compact proembryogenic cultures, GE globular embryos, HE heart-shaped embryos, TE torpedo-shaped embryos, CE cotyledonary embryos, ME mature embryos. Morphology of these embryogenic cultures has been described in previous studies (Lai et al. 2012; Lai and Lin 2013)