Fig. 4
Effect of temperature on the GTPase activity (a) and the stability (b) of the recombinant FtsZ. The effect of temperature on GTPase activity of the recombinant FtsZ was determined by the GTPase activity assay with 5 mM GTP in buffer solution (pH 7.0) as substrate at temperature range of 20–70 °C, thermal stability of the recombinant FtsZ was determined by measuring the retained GTPase activity after incubation at 4–60 °C for 240 min. The relative GTPase activity of the recombinant FtsZ is the ratio of the GTPase activity at certain temperature and at optimum temperature (a), the retained GTPase activity of the recombinant FtsZ is the ratio of the GTPase activity after and before treatment (b)