Fig. 1

Analysis of the process for FtsZ expression and purification by SDS-PAGE (a) and western blotting analysis of the purified FtsZ (b). The FtsZ was expressed in E. coli BL21 (DE3)/pET22b-ftsZ induced by 0.6 mM IPTG at 37 °C for 3 h. Lane M₁ and M₂ protein Marker; lane 1 purified FtsZ via Ni-NTA agarose column; lane 2 supernatant after ultrasonication under induced conditions; lane 3 total protein under induced conditions; lane 4 supernatant of broth under induced conditions; lane 5 supernatant of lysed E. coli BL21 cells containing pET-22b-ftsZ; lane 6 purified FtsZ via Ni-NTA agarose column. The migration difference of the 44.3 kDa protein between (a) and (b) is due to gel electrophoresis with different pulse time