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Table 1 Hatching rate, induction rate, abnormal rate and production rate for induced tetraploid at various treatment conditions in Korean rose bitterling, Rhodeus uyekii

From: Cytogenetic study of diploid and induced tetraploid in Korean rose bitterling, Rhodeus uyekii

Time after fertilization (min)

Duration of shock (min)

Water pressure (psi)

Rate (%)

Hatching

Induction

Abnormal

Production

Control

98 ± 1.2a

0a

0a

0

90

10

4500

74 ± 4.6b

0

0a

0

6000

48 ± 4.8b

6.0 ± 0.71a

1 ± 0.3b

2.9

7500

14 ± 5.1b

7.5 ± 0.55a

4 ± 0.2b

1.1

20

4500

68 ± 5.0c

0

1 ± 0.1b

0

6000

35 ± 3.8c

6.1 ± 0.66a

2 ± 0.1c

2.1

7500

4 ± 1.2c

7.8 ± 0.59a

5 ± 0.4c

0.3

30

4500

59 ± 3.9d

0

2 ± 0.4c

0

6000

21 ± 4.1d

6.4 ± 0.74a

3 ± 0.2d

1.4

7500

0

100

10

4500

75 ± 4.4b

0

0a

0

6000

48 ± 4.1b

6.2 ± 0.64a

1 ± 0.5b

3.1

7500

13 ± 4.5b

7.6 ± 0.55a

4 ± 0.3b

1.0

20

4500

66 ± 4.6c

0

1 ± 0.2b

0

6000

34 ± 5.4c

6.2 ± 0.53a

2 ± 0.2c

2.2

7500

6 ± 1.1c

7.9 ± 0.61a

5 ± 0.1c

0.5

30

4500

60 ± 4.1d

0

2 ± 0.1c

0

6000

18 ± 3.9d

6.3 ± 0.50a

3 ± 0.4d

1.2

7500

0

110

10

4500

73 ± 4.9b

0

0a

0

6000

47 ± 5.0b

6.1 ± 0.59a

1 ± 0.1b

2.9

7500

13 ± 4.9b

7.8 ± 0.72a

4 ± 0.2b

1.0

20

4500

68 ± 4.4c

0

1 ± 0.1b

0

6000

37 ± 4.4c

6.5 ± 0.77a

2 ± 0.5c

2.5

7500

5 ± 1.0c

7.9 ± 0.68a

5 ± 0.2c

0.4

30

4500

57 ± 4.0d

0

2 ± 0.3c

0

6000

20 ± 4.9d

6.6 ± 0.68a

3 ± 0.4d

1.3

7500

0

120

10

4500

74 ± 4.6b

0

0a

0

6000

45 ± 4.8b

6.2 ± 0.57a

1 ± 0.2b

2.8

7500

15 ± 4.3b

7.7 ± 0.65a

4 ± 0.4b

1.2

20

4500

68 ± 5.0c

0

1 ± 0.3b

0

6000

33 ± 3.8c

6.3 ± 0.81a

2 ± 0.3c

2.1

7500

4 ± 1.8c

8.0 ± 0.70a

5 ± 0.3c

0.3

30

4500

59 ± 3.9d

0

2 ± 0.2c

0

6000

19 ± 4.6d

6.6 ± 0.92a

3 ± 0.1d

1.3

7500

0

  1. Hatching rate = (the number of hatched eggs/the number of total eggs) × 100
  2. Induction rate = (the number of induced tetraploid/total number of treated eggs) × 100
  3. Abnormal rate = (the number of abnormal fries/the number of total fries) × 100
  4. Production rate = (hatched rate of treated eggs/hatched rate of control eggs) × induction rate of tetraploid
  5. Induction and production rate were measured at 2 days after hatched. Survival rate was measured at 10 days after hatched
  6. Each value is mean and standard deviation percentage of triplicate experiments (n = 30). Means in columns having different superscript letter are significantly different (P < 0.05)