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Table 1 Hatching rate, induction rate, abnormal rate and production rate for induced tetraploid at various treatment conditions in Korean rose bitterling, Rhodeus uyekii

From: Cytogenetic study of diploid and induced tetraploid in Korean rose bitterling, Rhodeus uyekii

Time after fertilization (min) Duration of shock (min) Water pressure (psi) Rate (%)
Hatching Induction Abnormal Production
Control 98 ± 1.2a 0a 0a 0
90 10 4500 74 ± 4.6b 0 0a 0
6000 48 ± 4.8b 6.0 ± 0.71a 1 ± 0.3b 2.9
7500 14 ± 5.1b 7.5 ± 0.55a 4 ± 0.2b 1.1
20 4500 68 ± 5.0c 0 1 ± 0.1b 0
6000 35 ± 3.8c 6.1 ± 0.66a 2 ± 0.1c 2.1
7500 4 ± 1.2c 7.8 ± 0.59a 5 ± 0.4c 0.3
30 4500 59 ± 3.9d 0 2 ± 0.4c 0
6000 21 ± 4.1d 6.4 ± 0.74a 3 ± 0.2d 1.4
7500 0
100 10 4500 75 ± 4.4b 0 0a 0
6000 48 ± 4.1b 6.2 ± 0.64a 1 ± 0.5b 3.1
7500 13 ± 4.5b 7.6 ± 0.55a 4 ± 0.3b 1.0
20 4500 66 ± 4.6c 0 1 ± 0.2b 0
6000 34 ± 5.4c 6.2 ± 0.53a 2 ± 0.2c 2.2
7500 6 ± 1.1c 7.9 ± 0.61a 5 ± 0.1c 0.5
30 4500 60 ± 4.1d 0 2 ± 0.1c 0
6000 18 ± 3.9d 6.3 ± 0.50a 3 ± 0.4d 1.2
7500 0
110 10 4500 73 ± 4.9b 0 0a 0
6000 47 ± 5.0b 6.1 ± 0.59a 1 ± 0.1b 2.9
7500 13 ± 4.9b 7.8 ± 0.72a 4 ± 0.2b 1.0
20 4500 68 ± 4.4c 0 1 ± 0.1b 0
6000 37 ± 4.4c 6.5 ± 0.77a 2 ± 0.5c 2.5
7500 5 ± 1.0c 7.9 ± 0.68a 5 ± 0.2c 0.4
30 4500 57 ± 4.0d 0 2 ± 0.3c 0
6000 20 ± 4.9d 6.6 ± 0.68a 3 ± 0.4d 1.3
7500 0
120 10 4500 74 ± 4.6b 0 0a 0
6000 45 ± 4.8b 6.2 ± 0.57a 1 ± 0.2b 2.8
7500 15 ± 4.3b 7.7 ± 0.65a 4 ± 0.4b 1.2
20 4500 68 ± 5.0c 0 1 ± 0.3b 0
6000 33 ± 3.8c 6.3 ± 0.81a 2 ± 0.3c 2.1
7500 4 ± 1.8c 8.0 ± 0.70a 5 ± 0.3c 0.3
30 4500 59 ± 3.9d 0 2 ± 0.2c 0
6000 19 ± 4.6d 6.6 ± 0.92a 3 ± 0.1d 1.3
7500 0
  1. Hatching rate = (the number of hatched eggs/the number of total eggs) × 100
  2. Induction rate = (the number of induced tetraploid/total number of treated eggs) × 100
  3. Abnormal rate = (the number of abnormal fries/the number of total fries) × 100
  4. Production rate = (hatched rate of treated eggs/hatched rate of control eggs) × induction rate of tetraploid
  5. Induction and production rate were measured at 2 days after hatched. Survival rate was measured at 10 days after hatched
  6. Each value is mean and standard deviation percentage of triplicate experiments (n = 30). Means in columns having different superscript letter are significantly different (P < 0.05)