Table 2 Method performance as measured in two consecutive days with three different concentrations of metabolites (low, medium and high)
Compound | Theoretical concentration ng mL−1 | Intraday precision (n = 5) (mean ± S.D.) | Interday precision (n = 10) (mean ± S.D.) | %RSD | %CV |
|---|---|---|---|---|---|
l-Amino adipic acid | 18.8 | 19.1 ± 3.1 | 19.2 ± 2.2 | 11.7 | 99.9 |
37.5 | 36.3 ± 2.1 | 39.1 ± 4.8 | 12.4 | 99.9 | |
75 | 75.9 ± 8.47 | 75.8 ± 6.5 | 10.5 | 100.0 | |
l-Saccharopine | 18.8 | 20.3 ± 2.8 | 19.1 ± 2.5 | 13.1 | 99.8 |
37.5 | 38.4 ± 3.8 | 37.5 ± 4.8 | 12.7 | 99.7 | |
75 | 71.9 ± 5.1 | 71.1 ± 5.9 | 12.3 | 99.8 | |
l-Pipecolic acid | 18.8 | 19.1 ± 1.4 | 19.1 ± 0.97 | 5.1 | 100.0 |
37.5 | 37.5 ± 0.8 | 37.4 ± 0.95 | 2.6 | 100.1 | |
75 | 76.4 ± 8.6 | 75.1 ± 4.3 | 5.7 | 100.0 | |
l-Glutamic acid | 18.8 | 18.7 ± 1.7 | 18.6 ± 1.6 | 8.6 | 100.0 |
37.5 | 35.4 ± 2.1 | 37.5 ± 3.1 | 8.3 | 99.9 | |
75 | 72.3 ± 5.7 | 74.7 ± 5.3 | 7.1 | 99.9 | |
l-Glutamine | 18.8 | 20.1 ± 3.8 | 19.5 ± 2.9 | 15.1 | 99.9 |
37.5 | 38.5 ± 4.3 | 37.6 ± 4.4 | 11.7 | 99.7 | |
75 | 73.7 ± 5.8 | 73.7 ± 8.7 | 8.8 | 99.9 | |
Pyridoxal phosphate | 18.8 | 18.2 ± 1.5 | 18.7 ± 1.6 | 8.4 | 104.0 |
37.5 | 36.2 ± 1.9 | 37.9 ± 2.7 | 7.1 | 100.0 | |
75 | 73.5 ± 6.1 | 75.7 ± 4.8 | 6.4 | 100.1 | |
Piperideine-6-carboxylic acid | 18.8 | 18.5 ± 1.31 | 19.5 ± 1.4 | 5.6 | 99.3 |
37.5 | 36.4 ± 1.2 | 37.53 ± 0.8 | 7.8 | 100.6 | |
75 | 74.1 ± 8.6 | 76.6 ± 5.4 | 7.9 | 99.9 |