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Table 2 Method performance as measured in two consecutive days with three different concentrations of metabolites (low, medium and high)

From: Simultaneous detection of lysine metabolites by a single LC–MS/MS method: monitoring lysine degradation in mouse plasma

Compound Theoretical concentration
ng mL−1
Intraday precision (n = 5)
(mean ± S.D.)
Interday precision (n = 10)
(mean ± S.D.)
%RSD %CV
l-Amino adipic acid 18.8 19.1 ± 3.1 19.2 ± 2.2 11.7 99.9
37.5 36.3 ± 2.1 39.1 ± 4.8 12.4 99.9
75 75.9 ± 8.47 75.8 ± 6.5 10.5 100.0
l-Saccharopine 18.8 20.3 ± 2.8 19.1 ± 2.5 13.1 99.8
37.5 38.4 ± 3.8 37.5 ± 4.8 12.7 99.7
75 71.9 ± 5.1 71.1 ± 5.9 12.3 99.8
l-Pipecolic acid 18.8 19.1 ± 1.4 19.1 ± 0.97 5.1 100.0
37.5 37.5 ± 0.8 37.4 ± 0.95 2.6 100.1
75 76.4 ± 8.6 75.1 ± 4.3 5.7 100.0
l-Glutamic acid 18.8 18.7 ± 1.7 18.6 ± 1.6 8.6 100.0
37.5 35.4 ± 2.1 37.5 ± 3.1 8.3 99.9
75 72.3 ± 5.7 74.7 ± 5.3 7.1 99.9
l-Glutamine 18.8 20.1 ± 3.8 19.5 ± 2.9 15.1 99.9
37.5 38.5 ± 4.3 37.6 ± 4.4 11.7 99.7
75 73.7 ± 5.8 73.7 ± 8.7 8.8 99.9
Pyridoxal phosphate 18.8 18.2 ± 1.5 18.7 ± 1.6 8.4 104.0
37.5 36.2 ± 1.9 37.9 ± 2.7 7.1 100.0
75 73.5 ± 6.1 75.7 ± 4.8 6.4 100.1
Piperideine-6-carboxylic acid 18.8 18.5 ± 1.31 19.5 ± 1.4 5.6 99.3
37.5 36.4 ± 1.2 37.53 ± 0.8 7.8 100.6
75 74.1 ± 8.6 76.6 ± 5.4 7.9 99.9
  1. The calculated concentrations, %RSD and %CV were obtained using MultiQuant 3.0.1 software (ABSciex)