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Table 1 LC-MS/MS method validation parameters

From: Simultaneous detection of lysine metabolites by a single LC–MS/MS method: monitoring lysine degradation in mouse plasma

Metabolite

Linear dynamic range (ηM)

Linear dynamic range (ηg mL−1)

Linear equation

R2

Retention time (min)

LOD (ηM)

LOQ (ηM)

l-Amino adipic acid

7.2–1860

1.17–300

y = 0.0911x + 0.0167

0.995

2.4

1.86

6.20

l-saccharopine

33.9–1085

9.3–300

y = 0.0215x + 0.0042

0.995

2.1

7.60

25.33

l-Pipecolic acid

9.07–2322

1.17–300

y = 0.0366x + 0.0008

0.999

2.9

2.32

7.74

l-Glutamic acid

7.96–2039

1.17–300

y = 0.0789x + 0.0545

0.995

2.2

2.03

6.11

Pyridoxal phosphate

37.9–1213

9.3–300

y = 0.0011x + 0.0020

0.996

4.8

14.97

36.4

Piperideine-6-carboxylic acid

9.2–2359

1.17–300

y = 0.0203x + 0.0053

0.998

2.4

2.35

19.6

  1. The linear equation represents the relation between the area ratio (peak area of analyte divided by peak area of IS) and ηg mL−1 concentration
  2. LOD limit of detection, LOQ limit of quantification
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