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Table 5 Real-time results after ezrin (Vil2) gene silencing

From: U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C

  

Exp 1

Exp 2

Exp 3

24 h

Vil2

Ud

Ud

Ud

48 h

Vil2

Ud

Ud

Ud

24 h

GAPDH

17,2933769

17,3035622

17,2984695

48 h

GAPDH

17,0784683

18,6328697

17,855669

24 h

PLC E

26,0919533

26,8768845

26,4844189

48 h

PLC E

25,7342033

27,7416077

26,7379055

24 h

PLC G2

31,3051376

30,6916809

30,9984093

48 h

PLC G2

30,8917274

31,3002968

31,0960121

24 h

PLC D4

30,2919121

30,5054932

30,3987026

48 h

PLC D4

30,415451

30,8388634

30,6271572

  1. Experiments were repeated each three times (exp 1, exp 2, exp 3). Transfected MG-63 cells (silencing of Vil2)
  2. Ud = undetected until 35 cycles