Table 1 PCR primers

PI-PLC β1 (PLCB1; OMIM *607120)

Forward 5′-AGCTCTCAGAACAAGCCTCCAACA-3′

Reverse 5′-ATCATCGTCGTCGTCACTTTCCGT-3′

PI-PLC β2 (PLCB2; OMIM *604114)

Forward 5′-AAGGTGAAGGCCTATCTGAGCCAA-3′

Reverse 5′-CTTGGCAAACTTCCCAAAGCGAGT-3′

PI-PLC β3 (PLCB3; OMIM *600230)

Forward 5′-TATCTTCTTGGACCTGCTGACCGT-3′

Reverse 5′-TGTGCCCTCATCTGTAGTTGGCTT-3′

PI-PLC β4 (PLCB4; OMIM *600810)

Forward 5′-GCACAGCACACAAAGGAATGGTCA-3′

Reverse 5′-CGCATTTCCTTGCTTTCCCTGTCA-3′

PI-PLC γ1 (PLCG1; OMIM *172420)

Forward 5′-TCTACCTGGAGGACCCTGTGAA-3′

Reverse 5′-CCAGAAAGAGAG CGTGTAGTCG-3′

PI-PLC γ2 (PLCG2; OMIM *600220)

Forward 5′-AGTACATGCAGATGAATCACGC-3′

Reverse 5′-ACCTGAATCCTGATTTGACTGC-3′

PI-PLC δ1 (PLCD1; OMIM *602142)

Forward 5′-CTGAGCGTGTGGTTCCAGC-3′

Reverse 5′-CAGGCCCTCGGACTGGT-3′

PI-PLC δ3 (PLCD3; OMIM *608795)

Forward 5′-CCAGAACCACTCTCAGCATCCA-3′

Reverse 5′-GCCA TTGTTGAGCACGTAGTCAG-3′

PI-PLC δ4 (PLCD4; OMIM *605939)

Forward 5′-AGACACGTCCCAGTCTGGAACC- 3′

Reverse 5′-CTGCTTCCTCTTCCTCATATTC- 3′

PI-PLC ε (PLCE; OMIM *608414)

Forward 5′-GGGGCCACGGTCATCCAC-3′

Reverse 5′-GGGCCTTCATACCGTCCATCCTC-3′

PI-PLC η1 (PLCH1; OMIM *612835

Forward 5′-CTTTGGTTCGGTTCCTTGTGTGG-3′

Reverse 5′-GGATGCTTCTGTCAGTCCTTCC-3′

PI-PLC η2 (PLCH2; OMIM *612836)

Forward 5′-GAAACTGGCCTCCAAACACTGCCCGCCG-3′

Reverse 5′-GTCTTGTTGGAGATGCACGTGCCCCTTGC-3′

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH)

Forward 5′-CGAGATCCCTCCAAAATCAA-3′

Reverse 5′-GTCTTCTGGGTGGCAGTGAT-3′