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Table 2 Open reading frames (ORFs) identified in the 7604-bp plasmid pRCEID7.6 from L. casei TISTR1341

From: Development of an Escherichia coliLactobacillus casei shuttle vector for heterologous protein expression in Lactobacillus casei

Gene/ORF

% GC content

5′ end position

3′ end position

No. of aa.

Known protein with the highest homology (microorganism)

% amino acid identity (length)

GenBank accession no.

repB

39

297

1076

259

Plasmid replication protein, RepB

100 (259)

YP_003329273.1

Plasmid pCD01p07 (L. paracasei subsp. paracasei)

  

ORF2

42

1461

1772

103

Hypothetical protein of plasmid pCD01p08 (L. paracasei subsp. paracasei)

97 (100)

YP_003329274.1

ORF3a

41

1609

2148

179

Putative replication protein of plasmid pCD01 (L. paracasei subsp. paracasei)

100 (179)

YP_003329275.1

ORF4a

36

2316

2684

122

Hypothetical protein LSEI_A19 of plasmid1 (L. casei ATCC334)

100 (122)

YP_796451.1

ORF5a

39

2977

3627

216

Hypothetical protein of plasmid1 (L. casei)

100 (216)

YP_005351865

ORF6a

38

3696

4886

396

C-terminus part of type IC specificity subunit protein (L. rhamnosus)

92 (180)

ZP_04439481.1

ORF7

43

6027

6377

116

Plasmid replication protein of pLR001 (L. rhamnosus HN001)

99 (115)

YP_002221586.1

ORF8a

45

6701

7237

178

Transposase (L. paracasei subsp. paracasei 8700:2)

96 (165)

ZP_04672204.1

ORF9a

45

7168

7488

106

Transposase tnp4 (L. casei BL23)

100 (106)

YP_002221607.1

Transposase (L. rhamnosus HN001)

100 (106)

YP_002221607.1

Transposase (L. buchneri ATCC 11577)

100 (106)

ZP_03943910.1

  1. aORFs are encoded on the complementary strand