Skip to main content

Table 2 Open reading frames (ORFs) identified in the 7604-bp plasmid pRCEID7.6 from L. casei TISTR1341

From: Development of an Escherichia coliLactobacillus casei shuttle vector for heterologous protein expression in Lactobacillus casei

Gene/ORF % GC content 5′ end position 3′ end position No. of aa. Known protein with the highest homology (microorganism) % amino acid identity (length) GenBank accession no.
repB 39 297 1076 259 Plasmid replication protein, RepB 100 (259) YP_003329273.1
Plasmid pCD01p07 (L. paracasei subsp. paracasei)   
ORF2 42 1461 1772 103 Hypothetical protein of plasmid pCD01p08 (L. paracasei subsp. paracasei) 97 (100) YP_003329274.1
ORF3a 41 1609 2148 179 Putative replication protein of plasmid pCD01 (L. paracasei subsp. paracasei) 100 (179) YP_003329275.1
ORF4a 36 2316 2684 122 Hypothetical protein LSEI_A19 of plasmid1 (L. casei ATCC334) 100 (122) YP_796451.1
ORF5a 39 2977 3627 216 Hypothetical protein of plasmid1 (L. casei) 100 (216) YP_005351865
ORF6a 38 3696 4886 396 C-terminus part of type IC specificity subunit protein (L. rhamnosus) 92 (180) ZP_04439481.1
ORF7 43 6027 6377 116 Plasmid replication protein of pLR001 (L. rhamnosus HN001) 99 (115) YP_002221586.1
ORF8a 45 6701 7237 178 Transposase (L. paracasei subsp. paracasei 8700:2) 96 (165) ZP_04672204.1
ORF9a 45 7168 7488 106 Transposase tnp4 (L. casei BL23) 100 (106) YP_002221607.1
Transposase (L. rhamnosus HN001) 100 (106) YP_002221607.1
Transposase (L. buchneri ATCC 11577) 100 (106) ZP_03943910.1
  1. aORFs are encoded on the complementary strand