Development of an Escherichia coli – Lactobacillus casei shuttle vector for heterologous protein expression in Lactobacillus casei

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Table 2 Open reading frames (ORFs) identified in the 7604-bp plasmid pRCEID7.6 from L. casei TISTR1341

Gene/ORF	% GC content	5′ end position	3′ end position	No. of aa.	Known protein with the highest homology (microorganism)	% amino acid identity (length)	GenBank accession no.
repB	39	297	1076	259	Plasmid replication protein, RepB	100 (259)	YP_003329273.1
repB	39	297	1076	259	Plasmid pCD01p07 (L. paracasei subsp. paracasei)
ORF2	42	1461	1772	103	Hypothetical protein of plasmid pCD01p08 (L. paracasei subsp. paracasei)	97 (100)	YP_003329274.1
ORF3^a	41	1609	2148	179	Putative replication protein of plasmid pCD01 (L. paracasei subsp. paracasei)	100 (179)	YP_003329275.1
ORF4^a	36	2316	2684	122	Hypothetical protein LSEI_A19 of plasmid1 (L. casei ATCC334)	100 (122)	YP_796451.1
ORF5^a	39	2977	3627	216	Hypothetical protein of plasmid1 (L. casei)	100 (216)	YP_005351865
ORF6^a	38	3696	4886	396	C-terminus part of type IC specificity subunit protein (L. rhamnosus)	92 (180)	ZP_04439481.1
ORF7	43	6027	6377	116	Plasmid replication protein of pLR001 (L. rhamnosus HN001)	99 (115)	YP_002221586.1
ORF8^a	45	6701	7237	178	Transposase (L. paracasei subsp. paracasei 8700:2)	96 (165)	ZP_04672204.1
ORF9^a	45	7168	7488	106	Transposase tnp4 (L. casei BL23)	100 (106)	YP_002221607.1
					Transposase (L. rhamnosus HN001)	100 (106)	YP_002221607.1
					Transposase (L. buchneri ATCC 11577)	100 (106)	ZP_03943910.1