Table 1 List of bacterial strains, plasmids, and oligonucleotide primers utilized in this study

Bacteria

 Escherichia coli XL-1 Blue

White/blue screening

Stratagene, La Jolla, CA

 Lactobacillus casei TISTR1341

Source of pRCEID7.6 native plasmid

TISTR

 Lactobacillus casei RCEID02

Plasmid-free L. casei TISTR1341-derivative

Panya et al. (2012)

 Lactobacillus casei ATCC393

Plasmid-free stain

ATCC

Plasmids

 pUC19E

Ap^r, Em^r, pUC19 carrying the Em^r gene from pE194 at the SmaI site

Leenhouts et al. (1991)

 pGEM-T easy

Ap^r, M13ori, T-overhang cloning vector

Promega, MD, USA

 pRCEID-LC2.9

Ap^r, Em^r, E. coli/L. casei shuttle vector based on pRCEID2.9

Panya et al. (2012)

 pRCEID-LC13.9Tc

Ap^r, Tc^r, pRCEID-LC13.9 derivative, the Tc^r gene was inserted at SacI site

Panya et al. (2012)

 pNZ8048

Cm^r, NcoI site used for translational fusions, standard vector

Mierau and Kleerebezem (2005)

Oligonucleotides

Sequence (5′–3′)

 p6.8F-1

gggtcagttttgccttatg

This study

 P6.8R-1

ctggcaatgactttgcgga

This study

 p6.8F

attgcttggatcctctggcatgacaaac (BamHI)

This study

 p6.8R

ctttttggtatacggatccagtcgcta (KpnI)

This study

 SpeI-ldh_F

caaactagtagcttttagtcctcgtgaaaa (SpeI)

Suebwongsa et al. (2013)

 pnisNdeI

attacatatgaagctcgcgttatcggtc (NdeI)

Suebwongsa et al. (2013)