Bacteria
|
Escherichia coli XL-1 Blue
|
White/blue screening
|
Stratagene, La Jolla, CA
|
Lactobacillus
casei TISTR1341
|
Source of pRCEID7.6 native plasmid
|
TISTR
|
Lactobacillus casei RCEID02
|
Plasmid-free L. casei TISTR1341-derivative
|
Panya et al. (2012)
|
Lactobacillus casei ATCC393
|
Plasmid-free stain
|
ATCC
|
Plasmids
|
pUC19E
|
Apr, Emr, pUC19 carrying the Emr gene from pE194 at the SmaI site
|
Leenhouts et al. (1991)
|
pGEM-T easy
|
Apr, M13ori, T-overhang cloning vector
|
Promega, MD, USA
|
pRCEID-LC2.9
|
Apr, Emr, E. coli/L. casei shuttle vector based on pRCEID2.9
|
Panya et al. (2012)
|
pRCEID-LC13.9Tc
|
Apr, Tcr, pRCEID-LC13.9 derivative, the Tcr gene was inserted at SacI site
|
Panya et al. (2012)
|
pNZ8048
|
Cmr, NcoI site used for translational fusions, standard vector
|
Mierau and Kleerebezem (2005)
|
Oligonucleotides
|
Sequence (5′–3′)
|
p6.8F-1
|
gggtcagttttgccttatg
|
This study
|
P6.8R-1
|
ctggcaatgactttgcgga
|
This study
|
p6.8F
|
attgcttggatcctctggcatgacaaac (BamHI)
|
This study
|
p6.8R
|
ctttttggtatacggatccagtcgcta (KpnI)
|
This study
|
SpeI-ldh_F
|
caaactagtagcttttagtcctcgtgaaaa (SpeI)
|
Suebwongsa et al. (2013)
|
pnisNdeI
|
attacatatgaagctcgcgttatcggtc (NdeI)
|
Suebwongsa et al. (2013)
|