Short-term assessment of BCR repertoires of SLE patients after high dose glucocorticoid therapy with high-throughput sequencing

Shi, Bin; Yu, Jiang; Ma, Long; Ma, Qingqing; Liu, Chunmei; Sun, Suhong; Ma, Rui; Yao, Xinsheng

doi:10.1186/s40064-016-1709-4

SpringerPlus

Table 3 Number of heavy chain variable regions that use the same V and J genes and have the same CDR3 length as known autoantibody from IMGT/LIGM-DB

From: Short-term assessment of BCR repertoires of SLE patients after high dose glucocorticoid therapy with high-throughput sequencing

Autoantibody accession no.	IGHVs	IGHJs	CDR3 length	P1-0	P1-1	P1-3	P2-0	P2-1	P2-3
AF035024	IGHV3-30	IGHJ4	6 AA	87	0	0	75	156	36
AF035021	IGHV3-30	IGHJ4	10 AA	1060	375	303	802	896	1353
L12105	IGHV1-2	IGHJ4	10 AA	14	36	38	136	379	227
AF035023	IGHV3-48	IGHJ3	11 AA	65	42	50	58	127	40
L12087	IGHV1-3	IGHJ4	11 AA	416	238	115	371	312	217
AF035020	IGHV3-48	IGHJ3	12 AA	235	0	198	95	183	96
AF035042	IGHV3-53	IGHJ4	12 AA	995	150	102	426	482	664
L12100	IGHV1-2	IGHJ6	12 AA	81	0	0	131	43	159
X54435	IGHV3-74	IGHJ5	12 AA	18	147	0	22	44	89
AF035018	IGHJ2-5	IGHJ4	13 AA	712	354	95	1471	850	792
AF035025	IGHV1-69	IGHJ2	13 AA	0	0	0	39	56	61
AF035041	IGHV4-59	IGHJ4	13 AA	961	248	362	1176	1393	1406
D16837	IGHV1-69	IGHJ4	13 AA	658	206	165	504	815	695
L12061	IGHV1-2	IGHJ4	13 AA	122	80	85	695	631	674
L12102	IGHV1-3	IGHJ4	13 AA	588	360	188	411	440	327
AF035043	IGHV3-43	IGHJ3	14 AA	76	35	77	131	114	126
L12090	IGHV3-53	IGHJ6	14 AA	180	0	113	148	84	116
L12098	IGHV1-3	IGHJ4	14 AA	743	419	138	598	437	449
X73856	IGHV1-8	IGHJ6	14 AA	88	0	0	55	99	160
AF035030	IGHV3-30	IGHJ4	15 AA	2939	1426	828	2052	2534	2339
S73912	IGHV3-21	IGHJ4	15 AA	5	0	0	0	0	0
M85255	IGHV3-30	IGHJ6	16 AA	1878	949	896	1225	1178	1138
U07194	IGHV1-2	IGHJ5	16 AA	50	0	24	163	84	85
AF035022	IGHV1-46	IGHJ4	17 AA	305	123	0	103	284	230
AF035040	IGHV6-1	IGHJ3	17 AA	0	0	162	36	19	0
L12096	IGHV1-69	IGHJ3	17 AA	44	33	23	175	167	164
U07196	IGHV4-61	IGHJ3	17 AA	0	0	0	0	0	0
X73851	IGHV1-2	IGHJ3	17 AA	0	29	5	147	93	126
X73857	IGHV4-31	IGHJ4	17 AA	160	98	204	0	0	0
AF035019	IGHV1-2	IGHJ3	18 AA	0	0	0	60	102	85
AF035027	IGHV3-9	IGHJ1	18 AA	0	0	0	8	24	8
X15611	IGHV1-18	IGHJ4	18 AA	382	154	13	300	277	228
D16833	IGHV3-74	IGHJ2	21 AA	0	0	0	0	0	29
X54445	IGHV4-4	IGHJ4	21 AA	0	0	0	0	0	0
X56592	IGHV4-34	IGHJ4	21 AA	265	95	122	321	170	371
X73859	IGHV3-23	IGHJ6	21 AA	517	200	75	403	583	329
AF035026	IGHV3-15	IGHJ4	22 AA	81	58	5	31	20	10
D84252	IGHV3-33	IGHJ6	22 AA	147	121	0	0	0	0
U07195	IGHV4-34	IGHJ5	22 AA	49	10	0	157	173	100
Total no.				13,921	5986	4386	12,525	13,249	12,929
Percent (%)				2.82	2.35	2.90	2.66	2.52	2.77

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