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Table 3 Standard curve of fluorescamine protein assay

From: Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer

Protein conc. (mg/ml)

BSA stock (ml)

Water (ml)

Fluorecamine (ml)

Incubation (min)

0

0

3.000

0.05

30

0.025

0.015

2.985

0.05

30

0.050

0.030

2.970

0.05

30

0.100

0.060

2.940

0.05

30

0.200

0.120

2.880

0.05

30

0.300

0.180

2.820

0.05

30