Table 2 Different elution agents and results
Group | Elution condition | Elution recovery of protein (%) | Elution recovery of activity (%) |
|---|---|---|---|
1 | 0.5 mol/L NaSCN | 56.30 | 54.01 |
2 | 1.0 mol/L NaSCN | 84.01 | 85.10 |
3 | 0.1 mol/L EDTA | 91.09 | 90.02 |
4 | 1 mol/L KSCN | 85.79 | 79.34 |
5 | pH7.0 PB + 0.5 mol/L Urea | 99.12 | 80.89 |
6 | pH7.0 PB +1.0 % Triton 100 | 17.85 | 10.98 |
7 | pH7.0 PB + 20.0 % Glycol | 89.96 | 85.09 |
8 | pH8.0 Gly–NaOH | 36.22 | 40.87 |
9 | pH9.0 Gly–NaOH | 56.50 | 61.40 |
10 | pH10.0 Gly–NaOH | 99.01 | 95.85 |