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Fig. 4 | SpringerPlus

Fig. 4

From: Separation of transglutaminase by thermo-responsive affinity precipitation using l-thyroxin as ligand

Fig. 4

Affinity precipitation of TGase. Initial concentration of TGase 2.0 mg/mL, 20.0 °C, pH 7.0, affinity ligand density 59.5 μmol/g, and 0.1 g polymer. a Effect of reaction time on adsorption on PNBN–thyroxin. b Effect of pH of PNBN–thyroxin on TGase binding. c Effect of ionic strength of PNBN–thyroxin on TGase binding. d Effect of ligand density on PNBN–thyroxin on TGase binding. e Adsorption isotherms of TGase binding to affinity polymer. Fitting plot of adsorption isotherm by Freundlich isotherm (blue line) and Langmuir–Freundlich isotherm (red line). Q e is the amount of TGase adsorbed per unit of the affinity polymer; C e is the equilibrium concentration of the TGase in the solution

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