Fig. 1From: Separation of transglutaminase by thermo-responsive affinity precipitation using l-thyroxin as ligandImmobilization of l-thyroxin to PNBN. l-Thyroxin was firstly reacted with the epichlorohydrin (ECH) in the NaOH solution (1 mol/L) at 60 °C for 24 h. Then the compounds were combined with PNBN through the hydroxyl groups at the conditions of 1 mol/L NaOH, 40 °C, 2 hBack to article page