Fig. 4From: ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent mannerAdministration of ZD7288 facilitated differentiation in a protocol-dependent manner. Relative expression levels of pluripotency genes (a) and a panel of differentiation genes (b) in cells spontaneously differentiated using FBS. Relative expression levels of pluripotency genes (c) and differentiation genes (d) using neurodifferentiation protocol. Ct values were normalized to Tbp expression. Student’s t-test; * p < 0.05; ** p < 0.01; *** p < 0.001, n.s. not significant; n = 3Back to article page