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Fig. 3 | SpringerPlus

Fig. 3

From: ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent manner

Fig. 3

HCN blocker treatment reduced colony formation frequency but did not induce differentiation. mESC plated at subclonal density were allowed to form colonies in control or ZD7288-containing media for 5 days and number of colonies per well was counted (Mann Whitney test; p < 0.05; n = 6) (a). Morphology was scored and fraction of rugged colonies calculated (Student’s t-test; p < 0.05; n = 6) (b). At the same time point, RNA was collected for qPCR analysis of pluripotency marker expression, which was unchanged (Student’s t-test; not significant; n = 3) (c). Finally, fixed cell were stained for pluripotency marker expression, with cells in both culture conditions showing expression (d)

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