Oxidative changes in the blood and serum albumin differentiate rats with monoarthritis and polyarthritis

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Table 4 Antioxidant capacity, thiol and carbonyl groups in the albumin molecule of control, mono- and poly-arthritic rats

Parameters	Units	Groups
Parameters	Units	Control	Monoarthritis	Polyarthritis
Albumin antioxidant capacity	μM (eq. trolox)	563.3 ± 52.0	531.2 ± 17.8	377.0 ± 7.0^#,*
Albumin antioxidant capacity	% of TAC	80	75	80
Albumin thiols groups	μM	188.7 ± 9.2	181.6 ± 6.2	85.1 ± 6.2^#,*
	% total thiol	82	78	71
	nmol/mg albumin	3.88 ± 0.27	3.33 ± 0.22	2.82 ± 0.18*
Albumin carbonyl groups	µM	92.3 ± 6.4	151.7 ± 3.2*	186.1 ± 7.6*^,#
	% total carbonyls	30	36	36
	nmol/mg albumin	2.20 ± 0.04	2.78 ± 0.13*	5.93 ± 0.60^#,*

The serum albumin separation was performed using affinity chromatography with Cibacron Blue 3G^® as described in the “Methods” section. The results are expressed in three ways: as μM, as nmol (mg albumin)⁻¹ and as the percentage of albumin parameters in relation to the total parameters before chromatography. Values are the mean ± SE of the mean of 3–4 samples for each experimental condition
* p < 0.05 indicates statistical difference from the controls and ^#p < 0.05 from the monoarthritic condition