Table 2 Experimental design using FCCCD of three independent variables with their actual and coded values and six centre points showing the experimental and predicted response
From: Cocoa pod husk, a new source of hydrolase enzymes for preparation of cross-linked enzyme aggregate
| Â | pH | Conc. (Mm) | Ratio (%w/v) | Lipase activity (U/ml) | |
|---|---|---|---|---|---|
Experimental | Predicted | ||||
1 | 7.00 (0) | 100.00 (0) | 6.00 (0) | 6.82 | 6.17 |
2 | 8.00 (+1) | 150.00 (+1) | 5.00 (−1) | 7.84 | 7.61 |
3 | 6.00 (−1) | 50.00 (−1) | 7.00 (+1) | 8.62 | 9.18 |
4 | 8.00 (+1) | 150.00 (+1) | 7.00 (+1) | 8.03 | 8.61 |
5 | 7.00 (0) | 100.00 (0) | 6.00 (0) | 6.79 | 6.17 |
6 | 8.00 (+1) | 100.00 (0) | 6.00 (0) | 9.23 | 8.98 |
7 | 8.00 (+1) | 50.00 (−1) | 5.00 (−1) | 10.02 | 10.59 |
8 | 6.00 (−1) | 150.00 (+1) | 7.00 (+1) | 9.01 | 8.91 |
9 | 8.00 (+1) | 50.00 (−1) | 7.00 (+1) | 11.43 | 11.26 |
10 | 7.00 (0) | 100.00 (0) | 6.00 (0) | 5.97 | 6.17 |
11 | 7.00 (0) | 100.00 (0) | 6.00 (0) | 6.82 | 6.17 |
12 | 6.00 (−1) | 100.00 (0) | 6.00 (0) | 7.48 | 7.58 |
13 | 6.00 (−1) | 50.00 (−1) | 5.00 (−1) | 7.93 | 7.49 |
14 | 7.00 (0) | 100.00 (0) | 5.00 (−1) | 4.93 | 4.89 |
15 | 7.00 (0) | 100.00 (0) | 7.00 (+1) | 6.70 | 6.24 |
16 | 6.00 (−1) | 150.00 (+1) | 5.00 (−1) | 6.58 | 6.89 |
17 | 7.00 (0) | 150.00 (+1) | 6.00 (0) | 6.84 | 6.50 |
18 | 7.00 (0) | 100.00 (0) | 6.00 (0) | 6.47 | 6.17 |
19 | 7.00 (0) | 50.00 (−1) | 6.00 (0) | 8.28 | 8.13 |
20 | 7.00 (0) | 100.00 (0) | 6.00 (0) | 5.84 | 6.17 |