Fig. 7
Map3k1 amiRNA reduced lung metastasis of 4T1 breast cancer cells. A The formation of lung tumor niches in control-amiRNA and Map3k1 amiRNA-overexpressing 4T1 cells after tail vein injection. Control-amiRNA 4T1 cells formed significantly higher numbers of tumor niches than Map3k1 amiRNA-overexpressing ones (arrowhead pointed to tumor niches). B HE staining was conducted to verify the tumor niches of the two groups (a, d, arrowhead indicated the region area of tumor niches formed by control cells were obviously larger than Map3k1 amiRNA-overexpressing cells; b, arrowhead pointed to the metastatic tumor formed by control 4T1 cells in blood vessel of lungs; c, arrowhead indicated inflammatory reaction and hyperplasia in lung tissue were occurring in control amiRNA group, whereas Map3k1 amiRNA-3 group maintained a integrated lung tissue)