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Fig. 2 | SpringerPlus

Fig. 2

From: MAP3K1-targeting therapeutic artificial miRNA suppresses the growth and invasion of breast cancer in vivo and in vitro

Fig. 2

Transfection of Map3k1 amiRNA significantly impaired cellular level of MEKK1 and downstream MAPK pathways in breast cancer cells. a Real-time PCR analysis of the interference efficiencies of different Map3k1 amiRNA constructs. b Time-dependence of MAP3K1 mRNA expression following Map3k1 amiRNA transfection using real-time PCR analysis. c Western blot detection of MEKK1 expression after Map3k1 amiRNA transfection. d The bar chart indicates the ratio of MEKK1 protein expression to GAPDH using densitometry after Map3k1 amiRNA transfection. e Determining the influence of Map3k1 amiRNA-3 overexpression on the levels of p-ERK, p-p38 and p-JNK MAPKs using western blot analysis. f Quantitative analysis of the intensities of MAPK protein bands relative to GAPDH in the indicated groups (*p < 0.05, **p < 0.01)

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