Fig. 3

TRIZ solution of CADMA PCR challenge. CADMA was performed with the same primer mixes, templates, and reaction mixtures as in Fig. 2. The interpretation windows (with orange lining) for primer mixes 1–4 are cycles 50–60, 30–45, 45, and 35, respectively. Primer mix 2 generated a concatenated artefact from the 50th cycle, primer mix 3 dimerized from the 45th cycle, while mix 4 dimerized from the 45th cycle and generated concatenated products from the 50th cycle. Though there is no universally suitable number of cycles for the four primer mixes and two DNA samples, TRIZ-derived PCR protocol enables a result for each primer mix after a single PCR run