Fig. 6

Identification of GFP-positive cells in the lung subjected to intratracheal instillation of GFP-expressing bone marrow cells. At 3 months after the intratracheal instillation of the bone marrow cells, bronchoalveolar lavage (BAL) was performed in the recipient mice, and flow cytometric analysis of surface antigens was performed on BAL cells. a The BAL fluid cells were classified as GFP-positive cells (group I) or GFP-negative cells (group II). Both GFP-positive BAL cells (group I) and GFP-negative BAL cells (group II) were F4/80+, Ly6G−, CD11c+, and weakly CD11b+. b The histogram shows that the expression patterns of F4/80, Ly6G, CD11c, and CD11b were identical in groups I and II. The red lines indicate the isotype control; the blue lines, the GFP-negative cells; and the yellow lines, GFP-positive cells (n = 3, with similar results). c The flow cytometric analysis of the bone marrow cells derived from the GFP mice revealed the staining patterns of CD11c, CD11b, Ly6g and F4/80 to be markedly different from those of the GFP-positive cells in the BAL cells. The red lines indicate the isotype control (n = 3, with similar results)