Fig. 5

Identification of GFP-positive cells in the lung subjected to intratracheal instillation of GFP-expressing lung cells. At 3 months after intratracheal instillation of the lung component cells, bronchoalveolar lavage was performed in the recipient mice, and flow cytometric analysis of the surface antigens was performed on the BAL cells. a After the exclusion of doublets and dead cells, the BAL fluid cells were classified as GFP-positive cells (group I) or GFP-negative cells (group II). The cells in group II are assumed to be host-specific residential macrophages that were originally present in the pulmonary alveoli. Groups I and II were F4/80+, Ly6G−, CD11c+, and weakly CD11b+. b The histogram shows that the expression patterns of F4/80, Ly6G, CD11c, and CD11b were identical in groups I and II. The red lines indicate the isotype control; the blue lines, GFP-negative cells; and the yellow lines, GFP-positive cells. The experiment was repeated 3 times (n = 3), yielding similar results