Fig. 3

The effect of mutolide on a TNF-α and IL-6 secretion LPS-stimulated THP-1 cells after 24 h. Values presented are average of n = 2. Mutolide showed dose-dependent inhibition of TNF-α and IL-6 with IC₅₀ being 1.27 ± 0.06 and 1.07 ± 0.02 µM, respectively. CC₅₀ for mutolide was 32.16 ± 1.53 µM, b TNF-α and IL-6 secretion from LPS-stimulated human peripheral blood mononuclear cells. Values presented are average of N = 3 donors. Mutolide had an IC₅₀ of 1.83 ± 0.33, 2.5 ± 0.5 and >100 µM for TNF-α inhibition, IL-6 inhibition and toxicity, respectively, c IL-17 secretion from anti-hCD3/anti-hCD28 co-stimulated hPBMCs and its effect on proliferation of anti-hCD3/anti-hCD28 stimulated and unstimulated hPBMCs. Values presented are average of N = 3 donors. Mutolide had an IC₅₀ of 0.63 ± 0.04, 4.36 ± 1.02 µM for IL-17 inhibition and proliferation of stimulated hPBMCs, respectively. All data are statistically analyzed by GraphPad Prism version 5.0. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001