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Table 2 The results of QCMD 2014 proficiency panel for C. pneumoniae and M. pneumoniae using the developed multiplex PCR method

From: Development of a multiplex real-time PCR assay for detection of Mycoplasma pneumoniae, Chlamydia pneumoniae and mutations associated with macrolide resistance in Mycoplasma pneumoniae from respiratory clinical specimens

Sample

Matrix

Sample content

Result with the multiplex-PCR

Sample status/percentage of laboratories that reported positive result for the pathogen in question

Sample type

CP.MP14-01

BAL

C. pneumoniae

C. pneumoniae

Frequently detected/96.2 %

Core

CP.MP14-02

TM

M. pneumoniae

M. pneumoniae

Detected/73.9 %

Educational

CP.MP14-03

TM

M. pneumoniae

Negative

Infrequently detected/20.6 %

Educational

CP.MP14-04

TM

Negative

Negative

Negative

Core

CP.MP14-05

TM

C. pneumoniae

C. pneumoniae

Frequently detected/98.4 %

Core

CP.MP14-06

TM

C. pneumoniae

Negative

Infrequently detected/58 %

Educational

CP.MP14-07

BAL

Negative

Negative

Negative

Core

CP.MP14-08

BAL

M. pneumoniae

M. pneumoniae

Detected/78.4 %

Educational

CP.MP14-09

TM

C. pneumoniae

C. pneumoniae

Detected/82.5 %

Educational

CP.MP14-10

BAL

C. pneumoniae

C. pneumoniae

Frequently detected/98.4 %

Core

CP.MP14-11

TM

M. pneumoniae

M. pneumoniae

Detected/94.5 %

Core

CP.MP14-12

BAL

M. pneumoniae

Negative

Infrequently detected/43.7 %

Educational

  1. BAL Bronchoalveolar lavage, TM transport medium, Core sample type that all participants are expected to detect correctly, Educational sample type that is designed to provide educational information to participants