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Fig. 1 | SpringerPlus

Fig. 1

From: Development of a multiplex real-time PCR assay for detection of Mycoplasma pneumoniae, Chlamydia pneumoniae and mutations associated with macrolide resistance in Mycoplasma pneumoniae from respiratory clinical specimens

Fig. 1

Melting curve displaying a patient sample containing DNA from M. pneumoniae with macrolide resistance-associated mutation (MR–MP). The melting profile of a patient sample was compared to the melting profiles of 3 controls: (1) Plasmid control 2063C which contains M. pneumoniae 23S rRNA PCR target sequence with base C at the position 2063 (DNA amplicon with base C at the position 2064 displays similar melting profile, data not shown). (2) Plasmid control 2063G which contains M. pneumoniae 23S rRNA target sequence with base G at the position 2063 (DNA amplicon with base G at position the 2064 displays similar melting profile, data not shown). (3) Wt (wild type) M. pneumoniae DNA

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