Table 2 Primers
From: Altered regulation of PDK4 expression promotes antiestrogen resistance in human breast cancer cells
Primer name | Length (BP) | Forward primer | Reverse primer |
|---|---|---|---|
18 s rRNA for qPCR | 149 | 5′ TCA ACT TTC GAT GGT AGT CGC CGT 3′ | 5′ TCC TTG GAT GTG GTA GCC GTT TCT 3′ |
β-actin mRNA for qPCR | 149 | 5′ GCC GCC AGC TCA CCA TGG AT 3′ | 5′ CAC CAT CAC GCC CTG GTG CC 3′ |
PDK4 mRNA for qPCR | 119 | 5′ CCT GTG AGA CTC GCC AAC A 3′ | 5′ TCC ACC AAA TCC ATC AGG CTC 3′ |
Cytochrome B genomic for qPCR | 125 | 5′-TGA TAT TTC CTA TTC GCC TAC ACA-3′ | 5′-TGT TGT TTG GAT ATA TGG AGG ATG-3′ |
Pyruvate kinase genomic for qPCR | 158 | 5′-GTC GAT CCA GGA GAA CAT ATC AT-3 | 5′-CTC CTA GTT TTC ACC CTC ATT TTC-3′ |
PDK4 genomic for sequencing | 367 | 5′ CAC TGA GAA TGT GAC CCG CT 3′ | 5′ AGC CTT GTG TGA AGT AAC CTT AG 3′ |
PDK4 cDNA for sequencing | 393 | 5′ ATC CTC CCG ACC CAA TTA GT 3′ | 5′ ACC CAC TGC TAC CAC ATC ACA 3′ |