Fig. 5

Tamoxifen resistant breast cancer cells have increased pyruvate dehydrogenase activity. On three consecutive days, lysates were prepared from identically treated MCF-7L and TamR-MCF-7 cells. Equal amounts of protein were subjected to a pyruvate dehydrogenase (PDH) assay. The average PDH activity expressed as NADH produced per minute per microgram of protein is shown for each assay replicate; error bars indicate one standard deviation (n = 3 biological replicates × 3 instrument replicates). The p value shown is from a two sided unpaired Student t test