Fig. 4

Adaptation to glucose deprivation increases resistance to doxorubicin without altering antiestrogen sensitivity. ATCC-MCF-7 cells were adapted to growth in glucose restricted media to generate MCF-7-GAL cells. a MCF-7-GAL and galactose naïve parental cells were treated with 1 nM ICI or control in media containing 4.5 mM glucose or 4.5 mM galactose. After 2 days, cell cycle phase distribution was determined by propidium iodide staining and flow cytometry. The average percent of cells in G1 phase (light gray), S phase (white) and G2/M phase (dark gray) are shown; error bars indicate one standard deviation (n = 3). b MCF-7-GAL and galactose naïve parental cells were treated with the indicated concentrations of doxorubicin for 24 h in media containing either 4.5 mM glucose or 4.5 mM galactose. Cellular mass was measured via sulforhodamine B (SRB) assay. The average SRB staining is shown relative to untreated cells; error bars indicate one standard deviation (n = 6)