Fig. 1

Glucose deprivation results in increased PDK4 protein without a change in mRNA. MCF-7L and TamR-MCF-7 cells were treated for 2 days in media containing 4.5 mM glucose or 4.5 mM galactose. a Total RNA was extracted, reverse transcribed, and the products used as template for qPCR amplification of PDK4 and b-actin. The average ratio is shown; error bars indicate one standard deviation (n = 3 biological replicates). b Whole cell lysates were prepared, and PDK4 and actin protein were visualized by western blotting, first for PDK4 (upper panel), then for actin (lower panel). The PDK4 band is indicated by a red arrow; background bands are indicated by black arrows. The actin band is indicated by a blue arrow