Table 1 Primer pairs and gene bank accession number of the gene used for the RT-qPCR assays
Genes | Primer pairs | Accession no |
|---|---|---|
Target genes | ||
p-38 MAPK | ||
F | GGC ACA CAG ACG ATG AGA TG | EF123661 |
R | ACA GCG TTC TGC CAG TGAG | |
TNFα | ||
F | GGC GAG CAT ACC ACT CCT CT | AY848945 |
R | TCG GAC TCA GCA TCA CCG TA | |
IL-1β | ||
F | GTA TCC CAT CAC CCC ATC AC | NM001123582 |
R | GCA AGA AGT TGA GCA GGT CC | |
Caspase 3 | ||
F | ACAGCA AAG AGC TAG AGG TCC AAC AC | DQ008070 |
R | AAA GCC AGG AGA CTT TGA CGC AG | |
NFkB | ||
F | CAG CGT CCT ACC AGG CTA AAG AGA T | CA341859 |
R | GCT GTT CGA TCC ATC CGC ACT AT | |
IL-8 | ||
F | GAGCGGTCAGGAGATTTGTC | NM_001140710 |
R | TTGGCCAGCATCTTCTCAAT | |
IL-6 | ||
F | ATG AAGGTT GCT GAG GTA GTG G | NM 001124657 |
R | TAG CAG TGT TGT CAT GGT TAC TGG | |
PGC-1α | ||
F | GTC AAT ATG GCA ACG AGG CTT C | FJ710605 |
R | TCG AAT GAA GGC AAT CCG TC | |
MnSOD | ||
F | GTT TCT CTC CAG CCT GCT CTA AG | DY718412 |
R | CCG CTC TCC TTG TCG AAG C | |
Catalase | ||
F | CCA GAT GTG GGC CGC TAA CAA | Est04a09 |
R | TCT GGC GCT CCT CCT CATT C | |
GPX-3 | ||
F | CCT TCC AGT ACC TGG AGT TGA ATG C | CA345853 |
R | CTC ATG ATT GTC TCC TGG CTC CTG T | |
Reference genes | ||
ELF1a | ||
F | TGC CCC TCC AGG ATG TCT AC | AF321836 |
R | CAC GGC CCA CAGGTA CTG | |
ARP | ||
F | GAA AAT CAT CCA ATT GCT GGA TG | AY255630 |
R | CTT CCC ACG CAA GGA CAG A | |