Fig. 4

The design chosen for the cell study. The primary liver cells and head kidney cells were grown in complete Leibowitz-15 media (cL-15) that either contained 20 % H-pro (H-pro solved in cL-15 media) or without H-pro supplementation (control). On day two 200 µM H₂O₂ was added or not to the cells with the aim to study the ability of H-pro to attenuate H₂O₂ induced oxidative stress and inflammation responses in primary liver and head kidney cells cultured as the respective monocultures and together in co-culture