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Fig. 2 | SpringerPlus

Fig. 2

From: Hydrolyzed fish proteins reduced activation of caspase-3 in H2O2 induced oxidative stressed liver cells isolated from Atlantic salmon (Salmo salar)

Fig. 2

From Western blots it was clear that activated cleaved caspase-3 was more profound in the co-cultured liver cells than in the corresponding mono-cultured liver cells (a). Lanes 1–4 are mono-cultured liver cells while lanes 5–8 is liver cells co-cultured with head kidney cells. Lanes 1 and 5 are controls, lanes 2 and 6 are cells grown in 20 % H-pro, lanes 3 and 7 are the control cells stressed with 200 µM H2O2 and lanes 4 and 8 are the cells grown in 20 % H-pro stressed with 200 µM H2O2. (b) The abundance means of activated cleaved caspase-3 relative to the abundance in the control that are set equal to 100 after the abundance was normalized. Cells grown with H-pro had less active cleaved caspase 3 as compared to controls (p = 0.024, Mann–Whitney U-test n = 5 ± SE)

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