Fig. 1From: Promoter methylation of TRIM9 as a marker for detection of circulating tumor DNA in breast cancer patientsPrimer designs for DNA methylation analysis of TRIM9 using NGS and for detection of TRIM9 methylated ctDNA using real-time PCR. Long primer sets were designed for DNA methylation analysis of TRIM9 by means of NGS (solid arrow). TRIM9 methylated ctDNA in plasma was detected by means of real-time PCR using the short primers (dashed arrow) and probes (dotted line)Back to article page