Table 3 Media composition for the transformation and regeneration of the japonica rice varieties Curinga and Nipponbare and the indica rice variety IR-64

CHU-Ind

Induction and proliferation of calli.

N6 salts and vitamins (Chu 1978), 100 mg l⁻¹ Myo-inositol, 2.5 mg l⁻¹ 2,4-d, 300 mg l⁻¹ Casein enzymatic hydrolysate, Maltose 30 g l⁻¹, Gelrite 3 g l⁻¹. Autoclave and supplement l-proline 500 mg l mg l⁻¹ and l-glutamine 500 mg l⁻¹. pH 5.8

CHU-Ind-AS

Co-cultivation

Chu-Ind medium supplemented with 100 µM acetosyringone. pH 5.8.

CHU-infection

Agro-Infection

N6 salts and vitamins (Chu 1978), 100 mg l⁻¹ Myo-inositol, 2,5 mg l⁻¹ 2,4-d, 1 g l⁻¹ Casamino acids, Maltose 15 g l⁻¹, Glucose 15 g l⁻¹. pH: 5.2. Filter sterilize. Add sterile acetosyringone 100 µM

CHU-H20

CHU-H40

CHU-H30

Selection

Chu-Ind medium supplemented with l-proline 500 mg l⁻¹, l-glutamine 500 mg l⁻¹, Gelrite 3 g l⁻¹, 20 mg l⁻¹ Hygromycin (for Curinga), 40 mg l⁻¹ Hygromycin (for Nipponbare), 30 mg l⁻¹ Hygromycin (for IR64), 250 mg l⁻¹ Cefotaxime. pH 5.8

MS-H20

MS-H40

MS-H30

Shoot induction/regeneration

MS salts and vitamins (Murashinge and Skoog 1962), 100 mg l⁻¹ Myo-inositol, 1 mg l⁻¹ NAA, 30 g l⁻¹ Sucrose, 3 g l⁻¹ Gelrite. Autoclave and supplement with 4 mg l⁻¹ Kinetin, 20 mg l⁻¹ Hygromycin (for Curinga), 40 mg l⁻¹ Hygromycin (for Nipponbare), 30 mg l⁻¹ Hygromycin (for IR64), and 250 mg l⁻¹ Cefotaxime. pH 5.8

MS-R-20

MS-R-40

MS-R-30

Rooting

MS salts and vitamins. 30 g l⁻¹ Sucrose, 3 g l⁻¹ Gelrite. Autoclave and supplement with, 20 mg l⁻¹ Hygromycin (for Curinga), 40 mg l⁻¹ Hygromycin (for Nipponbare), 30 mg l⁻¹ Hygromycin (for IR64), 250 mg l⁻¹ Cefotaxime. pH 5.8