Table 1 Summary table of the transformation events obtained by infecting rice varieties with A. tumefaciens (EHA105 and LBA4404) and E. adhaerens (OV14)
Bacterial strain | Rice variety | No. of infected calli | Plants delivered | PCR (+) | Southern blot (+) | 1C | 2C | 3C | Transformation efficiency (%) |
|---|---|---|---|---|---|---|---|---|---|
E. adhaerens | Nipponbare | 250 | 69 | 20 | 18 | 15 | 1 | 2 | 7.2 |
E. adhaerens | Curinga | 275 | 73 | 54 | 45 | 25 | 14 | 6 | 16 |
A. tumefaciens (EHA105) | Curinga | 25 | 14 | 11 | 8 | 7 | 1 | 0 | 32 |
A. tumefaciens (EHA105) | Nipponbare | 100 | 4 | 4 | 4 | 4 | 0 | 0 | 4 |
A. tumefaciens (LBA4404) | Curinga | 400 | 133 | 121 | 106 | 56 | 42 | 8 | 26.5 |
E. adhaerens | IR64 | 100 | 1 | 1 | 1 | 1 | – | – | 1 |