|
Substrates
|
V
max
|
K
s (mM)
|
S
in (mM)
|
R
2
|
|---|
|
l-Lactic acid
|
16.3 ± 1.2
|
6.7 ± 1.7
|
0.6 ± 0.3
|
0.94
|
|
Pyruvate (Na)
|
12.8 ± 0.9
|
3.7 ± 0.8
|
0.1 ± 0.1
|
0.96
|
|
Glycerol
|
7.1 ± 0.6
|
132 ± 34
|
10 ± 5
|
0.94
|
|
Glyoxylic acid
|
4.4 ± 0.2
|
47 ± 10
|
9 ± 3
|
0.95
|
|
Sucrose
|
4.3 ± 0.8
|
174 ± 82
|
21 ± 10
|
0.91
|
|
Mannitol
|
4.2 ± 1.1
|
296 ± 164
|
41 ± 16
|
0.93
|
|
Sorbitol
|
4.1 ± 0.4
|
231 ± 51
|
29 ± 5
|
0.97
|
|
Glucose
|
3.4 ± 0.2
|
105 ± 20
|
17 ± 4
|
0.96
|
|
Acetic acid
|
3.0 ± 0.6
|
0.43 ± 0.25
|
0.10 ± 0.06
|
0.84
|
|
Propionic acid
|
2.2 ± 0.2
|
0.24 ± 0.12
|
0.01 ± 0.05
|
0.84
|
|
Formic acid
|
1.8 ± 0.1
|
0.05 ± 0.03
|
0.03 ± 0.01
|
0.88
|
|
d-Lactic acid
|
1.8 ± 0.1
|
0.13 ± 0.07
|
0.1 ± 0.1
|
0.76
|
- Kinetic parameters were calculated by fitting to the modified Michaelis–Menten equation: \(v = V_{\text{max} } \left( {\left[ S \right] + \left[ {S_{\text{in}} } \right]} \right)/K_{\text{s}} + \left( {\left[ S \right] + \left[ {S_{\text{in}} } \right]} \right)\). V
max (nmol O2 [mg protein]−1 min−1) indicates maximal velocity of respiration. K
s indicates the substrate concentration which corresponds to the half of V
max. S means the concentration of exogenous substrate. S
in means hypothetical concentration of intracellular substrate. R
2 indicates coefficient of determination. Substrates are shown in descending order for the value of V
max. Each value is an average ± standard deviation of triplicates
