Substrates |
V
max
|
K
s (mM) |
S
in (mM) |
R
2
|
---|
l-Lactic acid | 16.3 ± 1.2 | 6.7 ± 1.7 | 0.6 ± 0.3 | 0.94 |
Pyruvate (Na) | 12.8 ± 0.9 | 3.7 ± 0.8 | 0.1 ± 0.1 | 0.96 |
Glycerol | 7.1 ± 0.6 | 132 ± 34 | 10 ± 5 | 0.94 |
Glyoxylic acid | 4.4 ± 0.2 | 47 ± 10 | 9 ± 3 | 0.95 |
Sucrose | 4.3 ± 0.8 | 174 ± 82 | 21 ± 10 | 0.91 |
Mannitol | 4.2 ± 1.1 | 296 ± 164 | 41 ± 16 | 0.93 |
Sorbitol | 4.1 ± 0.4 | 231 ± 51 | 29 ± 5 | 0.97 |
Glucose | 3.4 ± 0.2 | 105 ± 20 | 17 ± 4 | 0.96 |
Acetic acid | 3.0 ± 0.6 | 0.43 ± 0.25 | 0.10 ± 0.06 | 0.84 |
Propionic acid | 2.2 ± 0.2 | 0.24 ± 0.12 | 0.01 ± 0.05 | 0.84 |
Formic acid | 1.8 ± 0.1 | 0.05 ± 0.03 | 0.03 ± 0.01 | 0.88 |
d-Lactic acid | 1.8 ± 0.1 | 0.13 ± 0.07 | 0.1 ± 0.1 | 0.76 |
- Kinetic parameters were calculated by fitting to the modified Michaelis–Menten equation: \(v = V_{\text{max} } \left( {\left[ S \right] + \left[ {S_{\text{in}} } \right]} \right)/K_{\text{s}} + \left( {\left[ S \right] + \left[ {S_{\text{in}} } \right]} \right)\). V
max (nmol O2 [mg protein]−1 min−1) indicates maximal velocity of respiration. K
s indicates the substrate concentration which corresponds to the half of V
max. S means the concentration of exogenous substrate. S
in means hypothetical concentration of intracellular substrate. R
2 indicates coefficient of determination. Substrates are shown in descending order for the value of V
max. Each value is an average ± standard deviation of triplicates