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Fig. 3 | SpringerPlus

Fig. 3

From: Activation of oxidative carbon metabolism by nutritional enrichment by photosynthesis and exogenous organic compounds in the red alga Cyanidioschyzon merolae: evidence for heterotrophic growth

Fig. 3

Changes in gene expression in flat-plate culture. Transcript levels of genes related to glycolytic and oxidative pentose phosphate pathways, and Calvin–Benson and citric acid cycles were measured by quantitative RT-PCR. The peak of gene expressions during 0–6 h is color-coded. Explanatory color chart is shown at the lower right corner of this figure. Transcript level of SDH4 (succinate dehydrogenase hydrophobic subunit) was not determined because primer pair could not be designed by Primer Express software (Applied Biosystems) for crucially short and AT-rich sequence of SDH4 gene. Each gene name is shown on the arrows corresponding to enzymatic reaction. Orange and gray backgrounds show glycolytic and oxidative pentose phosphate pathways, respectively. Abbreviations of intermediates: 1,3BPG, 1,3-bisphoglycerate; 2OG, 2-oxoglutarate; 2PGA, 2-phosphoglycerate; 3PGA, 3-phosphoglycerate; 6PG, 6-phosphogluconate; AcCoA, acetyl-CoA; CIT, citrate; DHAP, dihydroxyacetone phosphate; E4P, erythrose 4-phosphate; F1,6BP, fructose 1,6-bisphosphate; F6P, fructose 6-phosphate; FUM, fumarate; G1P, Glucose 1-phosphate; G6P, glucose 6-phosphate; GAP, glyceraldehyde 3-phosphate; GLC, glucose; GLP, glucono 1,5-lactone 6-phosphate; ICIT, isocitrate; MAL, malate; OAA, oxaloacetate; PEP, phosphoenolpyruvate; PYR, pyruvate; R5P, ribose 5-phosphate; Ru5P, ribulose 5-phosphate; RuBP, ribulose 1,5-bisphosphate; S1,7BP, sedoheptulose 1,7-bisphosphate; S7P, sedoheptulose 7-phosphate; SUC, succinate; SucCoA, succinyl-CoA; Xu5P, xylulose 5-phosphate. Function of gene product: 2OGDH, 2-oxoglutarate dehydrogenase; 6PGDH, 6-phosphogluconate dehydrogenase; ACO, aconitase; CS, citrate synthase; ENO, enolase; FBA, fructose 1,6-bisphosphate aldolase; FBP, fructose 1,6-bisphosphatase; FUM, fumarase; G6PDH, glucose 6-phosphate dehydrogenase; GAPA, GAP dehydrogenase (NADP+ dependent, phosphorylating); GAPC, GAP dehydrogenase (NAD+ dependent, phosphorylating); GAPN, GAP dehydrogenase (NADP+ dependent, non-phosphorylating); GLK, glucokinase; ICDH, isocitrate dehydrogenase (NADP+ dependent); IDH, isocitrate dehydrogenase (NAD+ dependent); LPD (E3), pyruvate dehydrogenase E3/dihydrolipoamide dehydrogenase; MDH, malate dehydrogenase; ME, malic enzyme (NADP+ dependent); PDH, pyruvate dehydrogenase; PEPC, phosphoenolpyruvate carboxylase; PEPCK, phosphoenolpyruvate carboxykinase; PFK, phosphofructokinase (ATP dependent); PFP, phosphofructokinase (PPi dependent); PGAM, phosphoglycerate mutase; PGI, phosphoglucose isomerase; PGK, phosphoglycerate kinase; PGL, 6-phosphogluconolactonase; PGM, phosphoglucomutase; PK, pyruvate kinase; PRK, phosphoribulokinase; RBCL, ribulose bisphosphate carboxylase large subunit; RBCS, ribulose bisphosphate carboxylase small subunit; RPE, ribulose 5-phosphate 3-epimerase; RPI, ribose 5-phosphate isomerase; SBP, sedoheptulose 1,7-bisphosphatase; SCS, succinyl-CoA synthetase; SDH1, succinate dehydrogenase (Complex II) flavoprotein subunit; SDH2, succinate dehydrogenase iron-sulfur protein; SDH3, succinate dehydrogenase cytochrome B560 subunit; TKT, transketolase; TPI, triosephosphate isomerase

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