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Fig. 4 | SpringerPlus

Fig. 4

From: Embryonic and mature astrocytes exert different effects on neuronal growth in rat ventral mesencephalic slice cultures

Fig. 4

Co-culture of E14 VM and GFP-positive adult astrocytes. E14 VM-GFP-positive astrocytic co-cultures were evaluated at 21 DIV (ae). Neurons migrated on the vimentin-positive (VIM) astrocytic network originating from the VM tissue piece (a). Both the glial-associated nerve fibers (b) and non-glial-associated nerve fibers (c) grew in the presence of GFP-positive cells. GFP-positive cells had different morphology depending on their distance to the tissue piece, i.e. if they were closer to the tissue piece they were smaller with small processes (d), and when they were located further away from the tissue piece, they had polygonal shapes (e). TH = green, vimentin = blue, GFP-positive = red. Scale bars a = 100 µm, be = 50 µm

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