Fig. 3

Astrocytic and neuronal migrations in E18 cultures at different time points. E18 VM cultures were evaluated at 14 DIV (a), 21 DIV (b), 28 DIV (c), and 35 DIV (d). Vimentin-positive (VIM) astrocytes reached to significantly longer distances at 21 DIV, 28 DIV and 35 DIV compared to 14 DIV cultures (e). TH-positive neuronal migration was seen at all time points (a–d). White dots mark the circumference of the tissue piece. TH = Alexa 488, vimentin = Alexa 594, 14 DIV n = 17, 21 DIV n = 19, 28 DIV n = 8, 35 DIV n = 9, **p < 0.01, ***p < 0.001. Scale bar a–d = 100 µm