Skip to main content
Fig. 2 | SpringerPlus

Fig. 2

From: Embryonic and mature astrocytes exert different effects on neuronal growth in rat ventral mesencephalic slice cultures

Fig. 2

Astrocytic migration and nerve fiber outgrowth in E14 cultures at different time points. E14 VM cultures were fixed at 14 DIV (a), 21 DIV (b), 28 DIV (c), and 35 DIV (d). Vimentin-positive (VIM) astrocytes migrated significantly longer distances at 21 DIV compared to 14 DIV, and the distance was further strengthened at 28 DIV and 35 DIV (e). The glial-associated nerve fiber outgrowth was significantly increased at 21 DIV compared to 14 DIV, however, at 35 DIV it was shorter compared to 21 DIV cultures (f). The non-glial-associated nerve fiber outgrowth reached to longer distances at 21 DIV compared to 14 DIV (g). TH = Alexa 488, vimentin = Alexa 594, 14 DIV n = 10, 21 DIV n = 17, 28 DIV n = 11, 35 DIV n = 11, *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar ad = 100 µm

Back to article page