Opsonic function of sialic acid specific lectin in freshwater crab Paratelphusa jacquemontii

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Table 1 Hemagglutination activity (HA) of serum of clarified and purified lectin (Pjlec) with untreated and enzyme treated rabbit erythrocyte in Paratelphusa jacquemontii

S. no	Sample	Protein (mg/ml)	Hemagglutination activity (HA)
			Untreated rabbit erythrocyte	Enzyme treated rabbit erythrocyte
			Untreated rabbit erythrocyte	Trypsin^a	Neuraminidase^b	O-glycosidase^c
1	Serum	17.78 ± 2.35	2048	2048	4	2
2	Clarified serum	2.35 ± 0.67	2048	2048	4	2
3	Purified lectin	0.27 ± 0.14	2048	2048	4	2

Data represents median titer value from 10 determinations of serum, clarified serum and isolated lectin Pjlec against untreated and enzyme treated rabbit erythrocyte
Each sample (25 µl) serially diluted in Tris buffer saline (Tris: 50 mM, NaCl 100 mM, CaCl₂ 10 mM pH 7.5) and 1.5 % suspension of rabbit erythrocyte in same buffer was added and incubated for 1 h to observe hemagglutination titer
Control: untreated rabbit erythrocyte
Enzyme treated erythrocyte:
^aProteolytic enzyme -Trypsin (1 mg/ml) treated rabbit erythrocyte
^bAsialo rabbit erythrocyte : Neuraminidase (0.1 unit of Clostridium perfringens sialidase (Type X), in PBS-BSA (0.01 M sodium phosphate, and 0.145 M NaCl) pH 7, 37 °C for 4 h)
^cO-acetyl neuraminidase (O-glycosidase)