Fig. 6
From: Opsonic function of sialic acid specific lectin in freshwater crab Paratelphusa jacquemontii
Flow cytometry in vitro phagocytosis analysis of hemocyte population in the hemolymph of Paratelphusa jacquemontii. Flow cytogram of in vitro phagocytic activity observed in 100 µl hemocyte suspension in iso-osmotic buffer (Tris 50 mM, NaCl 210 mM, KCl 5 mM, MgCl2 2.5 mM, D-glucose 100 mg, pH 7.5, 480 mOsm) with glutaraldehyde fixed rabbit erythrocyte treated with enzymes and Pjlec. Mean SD: 94.6 2.1 (n 3). Scatter gram was generated by combining forward light scatter (FS) with 7-AAD fluorescence.a Untreated rabbit erythrocyte; b FITC labeled rabbit erythrocyte; c FITC labeled rabbit erythrocyte + hemocytes (2 min); d FITC labeled rabbit erythrocyte + hemocytes (30 min); e Lectin coated FITC labeled rabbit erythrocyte + hemocytes (2 min); f Pjlec lectin coated FITC labeled rabbit erythrocyte + hemocytes (30 min). g Trypsin treated FITC labeled rabbit erythrocyte + hemocytes (2 min); h Trypsin treated FITC labeled rabbit erythrocyte + hemocytes (30 min)