Fig. 5

Flow cytometry analysis of hemocyte population in the hemolymph of Paratelphusa jacquemontii. Hemolymph (100 µl) collected in 2 ml in iso-osmotic buffer (50 mM Tris, 210 mM NaCl, 5 mM KCl, 2.5 mM MgCl₂, 100 mg d-glucose, pH 7.5, 480 mOsm) scattered in flowcytogram in size (FSC) and granularity (SSC) as P1 granular hemocyte, P2 semigranular hemocyte and P3 hyaline hemocyte