Fig. 3From: Opsonic function of sialic acid specific lectin in freshwater crab Paratelphusa jacquemontii In vitro phagocytosis of untreated and serum, clarified serum and lectin Pjlec coated rabbit erythrocyte by hemocytes in the hemolymph of Paratelphusa jacquemontii. In vitro phagocytosis in percentage, % of rabbit erythrocyte suspended in iso-osmotic buffer, TBS: 50 mM Tris, 210 mM NaCl, 5 mM KCl, 2.5 mM MgCl2, 100 mg d-glucose, pH 7.5, 480 mOsm (control 16.48 %) or serum (92.35 mg ml−1) coated erythrocyte 17.54 %, clarified serum (2.3 mg ml−1) coated erythrocyte 19.50 % and lectin (0.04 mg ml−1) coated erythrocyte 35 % or anti lectin (3.5 mg ml−1) 17.55 %. Vertical bars represent mean ± SD of three determinations. Asterisk the variation by one way ANOVA in percentage of phagocytosis between control and clarified serum or lectin coated erythrocyte were statistically significant (p < 0.05). NS not significantBack to article page