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Fig. 3 | SpringerPlus

Fig. 3

From: Opsonic function of sialic acid specific lectin in freshwater crab Paratelphusa jacquemontii

Fig. 3

In vitro phagocytosis of untreated and serum, clarified serum and lectin Pjlec coated rabbit erythrocyte by hemocytes in the hemolymph of Paratelphusa jacquemontii. In vitro phagocytosis in percentage, % of rabbit erythrocyte suspended in iso-osmotic buffer, TBS: 50 mM Tris, 210 mM NaCl, 5 mM KCl, 2.5 mM MgCl2, 100 mg d-glucose, pH 7.5, 480 mOsm (control 16.48 %) or serum (92.35 mg ml−1) coated erythrocyte 17.54 %, clarified serum (2.3 mg ml−1) coated erythrocyte 19.50 % and lectin (0.04 mg ml−1) coated erythrocyte 35 % or anti lectin (3.5 mg ml−1) 17.55 %. Vertical bars represent mean ± SD of three determinations. Asterisk the variation by one way ANOVA in percentage of phagocytosis between control and clarified serum or lectin coated erythrocyte were statistically significant (p < 0.05). NS not significant

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