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Scheme 1

From: Binding interaction of a novel fluorophore with serum albumins: steady state fluorescence perturbation and molecular modeling analysis

Scheme 1

Reagent and conditions: a Conc. H2SO4, MeOH, 0 °C to r.t., 6 h. b Palladium (II) acetate (0.05 equiv.), xantphos (0.1 equiv.) and cesium carbonate (3 equiv.), 80 °C, 4 h. c Potassium tert-butoxide (1.2 equiv.), DMF, 0 °C to r.t., 12 h. d Lithium hydroxide (3 equiv.), MeOH–water (5:1), r.t., 2 h. e EDC.HCl (3.0 equiv.), HOBT (2.5 equiv.), TEA (6 equiv.), 0 °C to r.t., 1.5 h

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