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Table 2 Recovery of each hydroxybutyrate from human saliva

From: Simultaneous quantification of salivary 3-hydroxybutyrate, 3-hydroxyisobutyrate, 3-hydroxy-3-methylbutyrate, and 2-hydroxybutyrate as possible markers of amino acid and fatty acid catabolic pathways by LC–ESI–MS/MS

Hydroxybutyrate

Amount added (pmol)

Average recoverya [mean ± SD (n)] (%)

3HB

51.2

103.4 ± 3.6 (6)

103.2

104.6 ± 1.6 (6)

154.4

102.1 ± 1.1 (6)

3HIB

50.0

98.5 ± 2.0 (6)

100.0

99.7 ± 1.0 (6)

150.0

100.7 ± 1.1 (6)

3HMB

3.8

104.8 ± 1.5 (6)

7.6

107.7 ± 0.6 (6)

11.4

107.9 ± 0.9 (6)

2HB

19.2

108.8 ± 5.4 (6)

38.4

118.8 ± 2.2 (6)

57.6

108.7 ± 2.5 (6)

  1. Known amounts of each hydroxybutyrate were spiked into 5 µL of human saliva collected from healthy subjects before sample preparation
  2. aRecovery (%) = (amount found − \(\bar{X}_{0}\))/amount added × 100; \(\bar{X}_{0}\) value was obtained from Table 1
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