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Table 1 Reproducibility of the quantification of each hydroxybutyrate in human saliva

From: Simultaneous quantification of salivary 3-hydroxybutyrate, 3-hydroxyisobutyrate, 3-hydroxy-3-methylbutyrate, and 2-hydroxybutyrate as possible markers of amino acid and fatty acid catabolic pathways by LC–ESI–MS/MS

Hydroxybutyrate

Mean ± SD (n = 12) (pmol)

Relative SD

Sample preparation (%)

Error (SRM) (%)

3HB

56.0 ± 0.30

0.48

0.54

3HIB

49.0 ± 0.36

0.45

0.79

3HMB

3.7 ± 0.10

1.70

2.98

2HB

19.5 ± 0.84

5.28

3.45

  1. Each butyrate was quantified in 5 µL of saliva collected from healthy subjects. Four samples were prepared and quantified in triplicate by HPLC–MS/MS. The results were analyzed by a one-way layout, in which the analytical errors were divided into two sources: sample preparation and SRM measurement
  2. 3HB 3-hydroxybutyrate, 3HIB 3-hydroxyisobutyrate, 3HMB 3-hydroxy-3-methylbutyrate, 2HB 2-hydroxybutyrate
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